Polymorphism rs2275913 of Interleukin-17A is related to more intensive therapy with disease-modifying anti rheumatic drugs in Mexican patients with Rheumatoid Arthritis.

AIM: The study has two aims: 1) to evaluate the association of IL-17 polymorphism rs2275913 with RA severity and 2) to evaluate if this particular SNP is associated with susceptibility for RA in Mexican patients. METHODS: Seventy-six RA patients and ninety-four healthy controls were included in the study. RA patients were evaluated according to DAS 28. Treatment with DMARD'S was prescribed and radiological damage was evaluated according to the Larsen method. A case-control study was used. Oral epithelial cells were obtained as source for genetic material. DNA was amplified using PCR. Subsequently, a RFLP was carried out. Finally, in order to confirm the IL-17 SNP rs2275913 presence, direct sequencing of the DNA was performed. RESULTS: A significant difference was observed between the RA patients and controls when the prevalence of IL-17 SNP rs2275913 was compared. There was a statistically significant disparity among the two groups with an OR of 5.6 (95%CI 1.5 - 20.9, P=<0.01). In this study was observed that the RA patients who were positive for the IL-17 polymorphism rs2275913 required 3 DMARDs to control the disease compared to 32% of the patients who were negative for the IL-17 polymorphism rs2275913, OR 6.6 (95%CI 1.6 - 27.0, P<0.01). CONCLUSION: This study draws two main conclusions: 1) The presence of IL-17 polymorphism rs2275913 is closely related to a more severe form of the disease and as a result, a higher number of DMARDs required to control it, 2) The presence of IL-17 polymorphism rs2275913 may confer a risk of developing RA in Mexican carriers.

Anacusia súbita neurosensorial bilateral en un paciente con síndrome antifosfolípido primario microangiopático / Bilateral sudden sensorineural hearing loss in a patient with microangiopathic antiphospholipid syndrome

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Incremento en las concentraciones de ligando CD40 soluble plaquetario en pacientes con síndrome antifosfolipídico primario / Increased concentrations of soluble CD40 ligand platelet in patients with primary antiphospholipidic syndrome

Objetivo. Determinar las concentraciones de sCD40L en pacientes con SAFP, y su asociación con el número de trombosis. Pacientes y métodos. Se incluyó a pacientes con SAFP y controles sanos de la misma edad y sexo. Para su análisis, los pacientes con SAFP se dividieron en 2 grupos: a) pacientes con 1 trombosis, y b) pacientes > 1 trombosis. Se determinaron las concentraciones de sCD40L por método de ELISA en cada uno de ellos. Resultados. Las concentraciones de sCD40L fueron significativamente mayores en los pacientes con SAFP en comparación con el grupo control: 9,72 ± 11,23 ng/ml vs. 4,69 ± 40,04 ng/ml (p = 0,04). No hubo asociación entre las concentraciones séricas de sCD40L y el número de trombosis (9,81 ± 9,67 ng/ml en pacientes con una trombosis vs. 9,6 ± 12,75 ng/ml en ≥ 1 trombosis) (p = 0,13) En mujeres con embarazo y aborto del primer trimestre (13 pacientes), las concentraciones de sCD40L fueron mayores que en aquellas pacientes sin antecedente de aborto (26 pacientes), pero sin una diferencia estadística significativa (12,11 ± 16,46 ng/ml vs. 8,80 ± 8,61 ng/ml; p = 0,33). No se encontró correlación entre las concentraciones de sCD40L y el número total de trombosis. Conclusiones. Los pacientes con SAFP tienen concentraciones mayores de sCD40L en comparación con sujetos sanos, sin que esto se asocie a un mayor número de trombosis. Entre las pacientes con SAFP, existe una tendencia a mayores concentraciones de sCD40L en aquellas con embarazo e historia de aborto. Al ser la plaqueta la principal fuente celular de sCD40L, es posible que esta vía desempeñe un papel patogénico en la enfermedad (AU)

Objective. To determine the concentrations of sCD40L in patients with PAPS, and establish its association with the number of thrombosis. Patients and methods. We included patients with PAPS and healthy controls of the same age and sex. For analysis, patients with PAPS were divided into 2 groups: (1) patients with 1 thrombosis, and (2) patients with >1 thrombosis. Soluble CD40L concentrations were determined by ELISA method. Results. sCD40L concentrations were significantly higher in patients with PAPS compared with the controls (9.72±11.23 ng/ml vs 4.69±4.04 ng/ml) (P=.04) There was no association between serum levels of sCD40L and the number of thrombosis (1 thrombosis: 9.81±9.87 ng/ml vs 9.63±12.75 ng/ml in ≥1 thrombosis (P=.13). In women with pregnancy and abortions (13 patients), concentrations of sCD40L were higher than in those patients without a history of abortion (26 patients) but without statically significant difference (12.11±16.46 ng/ml vs 8.80±8.61 ng/ml) (P=.33). There was no correlation between levels of sCD40L and the total number of thrombosis. Conclusions. Patients with PAPS have higher concentrations of sCD40L compared with healthy subjects, although this is not associated with a greater number of thrombosis. Among patients with PAPS, there is a tendency to higher concentrations of sCD40L in women with pregnancy and history of abortion. Since the platelet is the main cellular source of sCD40L, is possible that this pathway plays a pathogenic role in patients with PAPS (AU)

Increased concentrations of soluble CD40 ligand platelet in patients with primary antiphospholipidic syndrome.

OBJECTIVE: To determine the concentrations of sCD40L in patients with PAPS, and establish its association with the number of thrombosis. PATIENTS AND METHODS: We included patients with PAPS and healthy controls of the same age and sex. For analysis, patients with PAPS were divided into 2 groups: 1) patients with 1 thrombosis, and 2) patients with >1 thrombosis. Soluble CD40L concentrations were determined by ELISA method. RESULTS: sCD40L concentrations were significantly higher in patients with PAPS compared with the controls (9.72 ng ± 11.23 ng/ml vs. 4.69 ± 4.04 ng/ml) (P=.04) There was no association between serum levels of sCD40L and the number of thrombosis (1 thrombosis: 9.81 ± 9.87 ng/ml vs 9.63 ± 12.75 ng/ml in ≥ 1thrombosis (P=.13). In women with pregnancy and abortions, (13 patients) concentrations of sCD40L were higher than in those patients without a history of abortion (26 patients) but without statically significant difference (12.11 ± 16.46 ng/ml vs. 8.80 ± 8.61 ng/ml) (P=.33). There was no correlation between levels of sCD40L and the total number of thrombosis. CONCLUSIONS: Patients with PAPS have higher concentrations of sCD40L compared with healthy subjects, although this is not associated with a greater number of thrombosis. Among patients with PAPS, there is a tendency to higher concentrations of sCD40L in women with pregnancy and history of abortion. Since the platelet is the main cellular source of sCD40L, is possible that this pathway plays a pathogenic role in patients with PAPS.